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Subsequent this, unilateral L5 spinal nerve ligation was executed. 7 times later, sensory thresholds have been reassessed and the development of allodynia to chilly and mechanical stimuli and hyperalgesia to your noxious thermal stimulus confirmed.

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A good response was recorded In the event the animal withdrew the paw pursuing software. For every measurement, the paw was sampled 5 situations along with a suggest calculated. No less than three min elapsed involving each test.

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